low salt buffer (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology low salt buffer
Low Salt Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 11767 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/low+salt+buffer/Triton+X-100/pmc12907709-85-16-8
Average 96 stars, based on 11767 article reviews

low salt buffer - by Bioz Stars, 2026-10

96/100 stars

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Incubation:

Article Title: Follicle-stimulating hormone-induced activation of Gata4 contributes in the up-regulation of Cyp19 expression in rat granulosa cells.
Article Snippet: After short spin supernatants were collected and 4–8 g of anti-GATA4 antibody (Santa Cruz; catalog no. sc-1237x) were added. .. After overnight incubation at 4 C, 50 l of protein A/G PLUS agarose were added, and the suspensions were rocked for another 2 h. Agarose beads were successively washed with low-salt buffer [0.1% SDS, 1% Triton X-100, 2 mM EDTA, 150 mM NaCl, 20 mM Tris-HCl (pH 8.1)], high-salt buffer [0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl (pH 8.1), 500 mM NaCl], ChIP wash buffer (Santa Cruz; catalog no. sc-45002), and a TE buffer [10 mM Tris (pH 8)/1 mM EDTA (pH 8)]. ..

Article Title: Zeb2 regulates the balance between retinal interneurons and Muller glia by inhibition of BMP–Smad signaling
Article Snippet: Sonicated material was brought to a final volume of 2 ml with ChIP dilution buffer (0.01% SDS, 1.1% Triton X-100, 1.2 mM EDTA, 17 mM Tris-HCl pH 8, 170 mM NaCl) and incubated with 15 μg anti-Zeb2 antibody (H260, Santa Cruz) or 10 μg anti-Smad1/5 antibody (D4G2, Cell Signaling Technologies) overnight at 4°C on a rotating wheel. .. The next day, protein A/G agarose beads (Santa Cruz) were added and incubated for 1 h. Beads were spun down at 1,000 gfor 1 min and washed as follows: once with low-salt buffer (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8.0, 150 mM NaCl) and transferred to nonstick low-binding 1.5-ml tubes, washed once with high-salt buffer (low-salt buffer with 500 mM NaCl), once with LiCl buffer (0.25 M LiCl, 1% NP-40, 1% sodium deoxycholate, 10 mM Tris-HCl pH 8.0, 1 mM EDTA), and twice with 10 mM Tris-HCl pH 8.0, 1 mM EDTA. ..

Article Title: A purine analog kinase inhibitor, calcium/calmodulin-dependent protein kinase II inhibitor 59, reveals a role for calcium/calmodulin-dependent protein kinase II in insulin-stimulated glucose transport.
Article Snippet: .. Thirty microliters of protein A-Sepharose [50% (wt/vol)] with 1–5 g anti-IR -subunit (CT-1 mouse monoclonal; gift from Dr. K. Siddle, University of Cambridge, Cambridge, UK) or anti-IRS1 (A-19 rabbit polyclonal; Santa Cruz) antibody were incubated with 400–500 g cell lysate and the mixture incubated overnight on a rotating wheel at 4 C. After washing immunoprecipitates once with 1 ml ice-cold high-salt buffer [PBS (pH 7.4) supplemented with 0.2% (vol/vol) Triton X-100 and 0.5 m NaCl] and twice with 1 ml ice-cold low-salt buffer [PBS (pH 7.4) supplemented with 0.2% (vol/vol) Triton X-100], samples were separated by SDS-PAGE [7.5% wt/vol) acrylamide] under reducing conditions. ..

Article Title: Zeb2 regulates the balance between retinal interneurons and Müller glia by inhibition of BMP-Smad signaling.
Article Snippet: Sonicated material was brought to a final volume of 2 ml with ChIP dilution buffer (0.01% SDS, 1.1% Triton X-100, 1.2 mM EDTA, 17 mM Tris-HCl pH 8, 170 mM NaCl) and incubated with 15 μg anti-Zeb2 antibody (H260, Santa Cruz) or 10 μg anti-Smad1/5 antibody (D4G2, Cell Signaling Technologies) overnight at 4 C on a rotating wheel. .. The next day, protein A/G agarose beads (Santa Cruz) were added and incubated for 1 h. Beads were spun down at 1000 gfor 1 min and washed as follows: once with low-salt buffer (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8.0, 150 mM NaCl) and transferred to non-stick low-binding 1.5-ml tubes, washed once with high-salt buffer (low-salt buffer with 500 mM NaCl), once with LiCl buffer (0.25 M LiCl, 1% NP-40, 1% sodium deoxycholate, 10 mM Tris-HCl pH 8.0, 1 mM EDTA), and twice with 10 mM Tris-HCl pH 8.0, 1 mM EDTA. ..

Chromatin Immunoprecipitation:

Sequencing:

Article Title: ELK3 Expression Correlates With Cell Migration, Invasion, and Membrane Type 1-Matrix Metalloproteinase Expression in MDA-MB-231 Breast Cancer Cells
Article Snippet: The cleared lysates were resuspended in IP buffer (167 mM NaCl, 16.7 mM Tris-HCl, pH 8.1, 1.2 mM EDTA, 0.01% SDS, and 1% Triton X-100) and immunoprecipitated with or without anti-ELK3 antibody [NET (C-20), sc-17860X; Santa Cruz Biotechnology, Inc.] by rotating overnight at 4°C. .. The immune complexes were recovered with protein A/G plus-agarose (sc-2003; Santa Cruz Biotechnology, Inc.) and washed in sequence with low-salt buffer (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl, pH 8.1 and 150 mM NaCl), high-salt buffer (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl, pH 8.1 and 500 mM NaCl), LiCl buffer (0.25 M LiCl, 1% NP40, 1% deoxycholate, 1 mM EDTA, and 10 mM Tris-HCl, pH 8.1), and TE buffer (10 mM Tris-HCl and 1 mM EDTA, pH 8.0) twice. ..

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low salt buffer (Santa Cruz Biotechnology)

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